Journal: Experimental Physiology
Article Title: Paternal sleep deprivation and cardiac dysfunction in offspring: A perspective of myocardial hypertrophy
doi: 10.1113/EP093212
Figure Lengend Snippet: Evaluation of reproductive health in mice after sleep deprivation. (a) Microscopic observation of sperm motility in the epididymal tail of mice in the control group and SD group ( n = 5 mice/group). (b) Observed sperm morphology in both groups using Haematoxylin and eosin staining ( n = 5 mice/group). (c) Measured serum testosterone concentrations in control mice and SD mice via ELISA ( n = 3–4 mice/group). (d) Measured serum corticosterone concentrations in control mice and SD mice via ELISA. n = 3–4 mice/group. Data are expressed as means ± SD and were analysed using an independent samples t ‐test. * P < 0.05 compared with control.
Article Snippet: Serum samples were collected from parental mice, and serum testosterone (Wuhan Fine Biotech Co., Ltd, Wuhan, China) and corticosterone (Jiangsu Meimian Industrial Co., Ltd, Yancheng, China) levels were assayed using an enzyme‐linked immunosorbent assay kit according to the manufacturer's instructions.
Techniques: Control, Staining, Enzyme-linked Immunosorbent Assay